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KMID : 0900920000240020199
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Korean journal of Animal Reproduction
2000 Volume.24 No. 2 p.199 ~ p.208
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The Study of Factor Concerning Ovum Pick-up and Conception Rate by Ultrasound-Guided Follicular Aspiration in Hanwoo Heifers
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Yang B.-S.
Seong Hwan-Hoo
Cheong I. C.
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Abstract
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This study was carried out to investigate of factors concerning to ultrasound-guided follicular aspiration; level of vacuum pressure, diameter of use needle, effect of FSH hormone and conception rate after embryos transfer. 1. Oocytes collection number were 4.2pm2.9 e.a to luteuml phase and follicular phase were 4.4pm3.5 e.a to ovaries of Hanwoo. 2. We taked proper level of aspiration vacuum pressure was 40~120 mmHg to oocytes collection. Oocytes collected number were 4.2pm3.2, 4.3pm3.4, 4.5pm3.4 e.a. to 40, 80, 120mmHg, respectively, follicles aspiration rate were 49, 47, 45%. 3. Effect of collection needle diameter was not difference significantly(P＜0.05), oocytes collected number were 4.4pm3.5 e.a to 170 and 3.0pm1.8 e.a to 18G needle, collected oocytes quality were no difference significantly (P＜0.05). 4. Follicles increase number to FSH hormone injection were 6.2pm2.3 e.a to intramuscle and 1.1pm2.7 e.a. to epithelial injection method. 5. Conception rate derived from E.T. was 11.1% to freezing embryos and 46.2% to fresh E.T., difference significantly(P＜0.05).
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KEYWORD
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Ultrasound-guided follicular aspiration, Conception rate, E.T.
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